《植物生理学报》 2018, 54(5): 845-854

黑大豆根系对溶液甲醛的吸收及其代谢产物分析

陈悦^#, 谭浩^#, 郭红霞, 冯泳, 陈丽梅^*

昆明理工大学生命科学与技术学院生物工程技术研究中心, 昆明650500

通信作者：陈丽梅；E-mail: chenlimeikm@126.com

摘　要：

本研究考查在2、4和6 mmol·L^-1甲醛(HCHO)溶液中处理的‘丹波’黑大豆(Glycine max cv. Tamba)根系对HCHO的吸收与代谢产物。结果表明, 在48 h处理期内根系对HCHO的吸收与时间的关系为指数函数。活体根系吸收HCHO对其去除的贡献率分别为30.1%、26.7%和23.0%, 显著大于死根系对HCHO的吸附作用, 这一结果表明活体根中发生了HCHO代谢作用。碳-13核磁共振(¹³C-NMR)分析表明, 根系吸收的H¹³CHO首先被氧化为H¹³COOH; 在H¹³CHO处理0~4 h期间, H¹³COOH被同化为草酸、甘氨酸、天冬酰胺、谷氨酰胺和丝氨酸, 在4~48 h期间, 这些有机酸被转化为苹果酸、柠檬酸、异柠檬酸和葡萄糖。4和6 mmol·L^-1 H¹³CHO处理2 h的根系中代谢产物种类与2 mmol·L^-1处理的相似, 但每种代谢产物产量显著提高。

关键词：甲醛去除; 甲醛吸收; 甲醛代谢; ‘丹波’黑大豆根

收稿：2017-10-23   修定：2018-02-27

资助：国家自然科学基金(31560071)。

Absorption of formaldehyde by black soybean roots and products of formaldehyde metabolism

CHEN Yue^#, TAN Hao^#, GUO Hong-Xia, FENG Yong, CHEN Li-Mei^*

Biotechnological Engineering Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

Corresponding author: CHEN Li-Mei; E-mail: chenlimeikm@126.com

Abstract:

It has been shown that plant roots have considerable contribution to formaldehyde (HCHO) removal in bioreactors, which is associated with HCHO assimilation by roots. This study investigates HCHO absorption and metabolites in the roots of ‘Tamba’ black soybean (Glycine max) treated in 2, 4 and 6 mmol·L^-1 HCHO solutions. The results show that the relationship between HCHO absorption and treatment time over a 48 h treatment period fits an exponential function. The contribution (30.1%, 26.7% and 23.0%) of HCHO absorption by living roots to HCHO removal were significantly greater than that of the HCHO adsorption by dead roots after treatment for 48 h. The evidence suggests the presence of HCHO metabolism is in the living roots. ¹³C-NMR (carbon-13 nuclear magnetic resonance) analyses indicated that, in 2 mmol·L^-1 H¹³CHO treated-roots, the absorbed H¹³CHO was first oxidized to H¹³COOH, which was then mainly converted to oxalate, glycine, asparagine, glutamine and serine during the 0-4 h treatment period. During the 4-48 h treatment period, these organic acids were converted to malate, citrate, isocitrate and glucose. The metabolite types in 4 and 6 mmol·L^-1 H¹³CHO treated-roots for 2 h were similar to those in 2 mmol·L^-1 H¹³CHO treated-roots but the yield of each metabolite was significantly increased.

Key words: formaldehyde removal; formaldehyde absorption; formaldehyde metabolism; ‘Tamba’ black soybean roots